Single molecule loading methods and compositions

US8906831B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• John Eid · San Francisco, US
• Stephen Turner · Menlo Park, US
• Ravi Kumar Dalal · Dallas, US
• Benjamin Flusberg · Atlanta, US
• Jonas Korlach · Camas, US
• Steven Lin · Palo Alto, US
• Adrian Fehr · San Francisco, US
• Fred Christians · Los Altos Hills, US
• Robin Emig · Belmont, US
• Jeremy Gray · Larkspur, US
• Robert Sebra · San Francisco, US
• Lei Sun · Santa Clara, US
• Stephen Dudek · San Francisco, US

Key dates

• Filing date: Mar 30, 2009
• Grant date: Dec 9, 2014
• Expiry date: Oct 1, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/06
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.