Method for analyzing secretome, biomarker for lung cancer metastasis, and siRNA compound for inhibiting lung cancer metastasis
US8912155B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 27, 2011 |
| Grant date | Dec 16, 2014 |
| Priority date | — |
| Expiry date | Sep 17, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/7028
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for analyzing secretome, a biomarker for lung cancer metastasis, and a siRNA compound for inhibiting lung cancer metastasis are disclosed. The method for analyzing secretome of the present invention comprises the following steps: (A) collecting proteome secreted from a cell; (B) providing a purification gel, wherein the purification gel comprises a low-density layer, and a high-density layer, and the low-density layer is stacked on the high-density layer; (C) adding the proteome on the low-density layer, and separating the proteome through the low-density layer and the high-density layer of the purification gel; (D) collecting the separated proteome on the interface between the low-density layer and high-density layer, and tagging the separated proteome with a reagent after digestion; and (E) analyzing the separated proteome tagged with the reagent, and comparing an analysis result with a proteomic database.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.