Methods for enhancing nucleic acid hybridization

US8916345B2 · kind B2 · utility

Assignee

• INTEGRATED DNA TECHNOLOGIES, INC. · US

Inventors

• Scott Rose · Coralville, US
• Mark Aaron Behlke · Coralville, US
• Richard Owczarzy · Coralville, US
• Joseph A. Walder · Iowa City, US
• Derek M. Thomas · Cedar Rapids, US
• Michael R. Marvin · Coralville, US

Key dates

• Filing date: Mar 28, 2011
• Grant date: Dec 23, 2014
• Expiry date: Apr 5, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6851
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A composition comprising an oligonucleotide having the structure 5′-Y1-L1-X-L2-Y2-3′. Y1 comprises a sequence of one or more DNA or RNA nucleotides, including a first nucleotide N1 having a 3′ phosphate covalently linked to L1. Y2 comprises a sequence of one or more DNA or RNA nucleotides, including a second nucleotide N2 having a 5′ phosphate covalently linked to L2. L1 and L2 each independently are a direct bond or a C1-C7 alkyl, alkynyl, alkenyl, heteroalkyl, substituted alkyl, aryl, heteroaryl, substituted aryl, cycloalkyl, alkylaryl, or alkoxyl group. X isR1 is a hydrogen or a C1-C8 alkyl. M is a label or ligand comprising a fused polycyclic aromatic moiety.