Reagents and methods for direct labeling of nucleotides
US8916346B2 · kind B2 · utility
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Key dates
| Filing date | Dec 6, 2011 |
| Grant date | Dec 23, 2014 |
| Priority date | — |
| Expiry date | Dec 6, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6841
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.