PCR reaction mixtures with decreased non-specific activity
US8916352B2 · kind B2 · utility
9Cited by
8References
10Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 10, 2012 |
| Grant date | Dec 23, 2014 |
| Priority date | — |
| Expiry date | Jun 6, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.