Dynamic monitoring of activation of receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US8916357B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 7, 2012 |
| Grant date | Dec 23, 2014 |
| Priority date | — |
| Expiry date | Aug 7, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control to at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.