Methods of RNA display
US8916504B2 · kind B2 · utility
2Cited by
10References
35Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 30, 2009 |
| Grant date | Dec 23, 2014 |
| Priority date | — |
| Expiry date | Oct 3, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1041
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.