DNA polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 · kind B2 · utility
1Cited by
8References
8Claims
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Key dates
| Filing date | Apr 8, 2014 |
| Grant date | Dec 30, 2014 |
| Priority date | — |
| Expiry date | Apr 8, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are described to modify Family B DNA polymerases that contain residual exonuclease activity that interferes with sequencing techniques and with detection of single nucleotide polymorphisms. The compositions are mutant proteins with reduced exonuclease activity compared with presently available “exo−” polymerases, and a sensitive screening assay that enables an assessment of exonuclease activity of any synthetic DNA polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.