Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants

US8921281B2 · kind B2 · utility

Assignee

• Novimmune SA · CH

Inventors

• Nicolas Fischer · Geneva, CH
• Marie Kosco-Vilbois · Saint-Julien-en-Genevois, FR
• Ulla Ravn · Geneva, CH
• Franck Gueneau · Saint-Julien-en-Genevois, FR
• Sophie Venet-Bonnot · Saint-Julien-en-Genevois, FR

Key dates

• Filing date: May 20, 2010
• Grant date: Dec 30, 2014
• Expiry date: Dec 31, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/622
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides compositions and methods for generating libraries of DNA sequences encoding homologous polypeptides, and uses of the libraries to identify naturally diversified polypeptide variants. The invention also provides compositions and methods for generating collections of synthetic antibody fragments in which one or several complementary determining regions (CDR) are replaced by a collection of the corresponding CDR captured from a natural source. The invention further provides compositions and methods for diversifying a portion of a polypeptide by inserting a diversified sequence of synthetic or natural origin without the need for modification of the original polypeptide coding sequence.