Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
US8927218B2 · kind B2 · utility
108Cited by
2References
29Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 26, 2012 |
| Grant date | Jan 6, 2015 |
| Priority date | — |
| Expiry date | Jul 20, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods, compositions and kits for segregating a target nucleic acid from a mixed nucleic acid sample. The methods, compositions and kits comprise a non-processive endonuclease (e.g., a restriction enzyme) or an antibody that binds the target nucleic acid (e.g., has methylation specificity). The mixed nucleic acid sample can comprise prokaryotic and eukaryotic nucleic acid and/or nucleic acid from more than one prokaryotic or eukaryotic organisms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.