Patent · US Active

C-terminal modification of polypeptides

US8927230B2 · kind B2 · utility

1Cited by

2References

4Claims

0Family size

Assignee

Eucodis Bioscience GmbH · AT

Inventors

Eva Hoess · Starnberg, DE
Frank Bordusa · Koblenzer Straße, DE
Rupert Hermann · Weilheim, DE
Hans-Dieter Jakubke · Leipzig, DE
Wilhelm Tischer · Peißenberg, DE

Key dates

Filing date	Jan 14, 2013
Grant date	Jan 6, 2015
Priority date	—
Expiry date	Jan 14, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P21/06
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaa1-Xaa2-His, wherein Xaa1 is L, Y or F and Xaa2 is R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.