Separatome-based protein expression and purification platform

US8927231B2 · kind B2 · utility

Assignees

• THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS · US
• University of Pittsburgh—Of the Commonwealth System of Higher Education · US

Inventors

• Ellen M. Brune · Fayetteville, US
• Robert Beitle · Fayetteville, US
• Mohammad M. Ataai · Pittsburgh, US
• Patrick R. Bartlow · Pittsburgh, US
• Ralph Henry · Fayetteville, US

Key dates

• Filing date: Oct 17, 2013
• Grant date: Jan 6, 2015
• Expiry date: Oct 17, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.