Patent · US Active

I-CreI derived single-chain meganuclease and uses thereof

US8927247B2 · kind B2 · utility

0Cited by

27References

10Claims

0Family size

Assignee

CELLECTIS SA · FR

Inventor

Sylvestre Grizot · Jacou, FR

Key dates

Filing date	Feb 2, 2009
Grant date	Jan 6, 2015
Priority date	—
Expiry date	Sep 3, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

A new I-CreI derived single-chain meganuclease comprising two domains, each domain comprising a portion of a parent I-CreI monomer which extends at least from the beginning of the first alpha helix to the end of the C-terminal loop and said two domains being joined by a peptidic linker which allows them to fold as a I-CreI dimer that is able to bind and cleave a chimeric DNA target comprising one different half of each parent homodimeric I-CreI meganuclease target sequence. Use of said I-CreI derived single-chain meganuclease for genetic engineering, genome therapy and antiviral therapy.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.