Derivatization of PNGase F released glycans on an HPLC chip
US8927263B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Jul 29, 2013 |
| Grant date | Jan 6, 2015 |
| Priority date | — |
| Expiry date | Jul 29, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2400/38
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.