Rapid membrane isolation method for animal and plant samples
US8927268B2 · kind B2 · utility
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6Claims
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Key dates
| Filing date | Feb 14, 2013 |
| Grant date | Jan 6, 2015 |
| Priority date | — |
| Expiry date | Feb 14, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M47/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for quickly obtaining crude membranes from cells, including animal and plant cells. The methods include incubating cells in a buffer and forcing the cells through a filter that causes rupture of the cells, and then separating the resulting crude membranes from most cytosolic proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.