Method to engineer mammalian-type carbohydrate structures
US8932825B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 24, 2002 |
| Grant date | Jan 13, 2015 |
| Priority date | — |
| Expiry date | Jan 5, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01024
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to host cells having modified lipid-linked oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells have a GlcNAcMan3GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyl-transferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.