Patent · US Active

Insertion of sequence elements into nucleic acids

US8932831B2 · kind B2 · utility

11Cited by

4References

39Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Christian Korfhage · Hilden, DE
Holger Engel · Hilden, DE
Dirk Löffert · Mülheim an der Ruhr, DE
Ralf Peist · Hilden, DE

Key dates

Filing date	May 4, 2007
Grant date	Jan 13, 2015
Priority date	—
Expiry date	May 7, 2027

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6816
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention concerns a method for inserting one or more tag sequences into a nucleic acid characterized by the following steps: (a) preparation of a template nucleic acid; (b) hybridization of at least one anchor sequence of at least one anchor oligonucleotide with one sequence section of the template nucleic acid; and (c) synthesization of a new strand of nucleic acid, which is partially complementary to the template nucleic acid and which contains a sequence complementary to the non-hybridized portion of the anchor oligonucleotide, e.g. to at least one tag sequence, on its 3′ end.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.