High throughput multichannel reader and uses thereof
US8936762B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 1, 2010 |
| Grant date | Jan 20, 2015 |
| Priority date | — |
| Expiry date | May 3, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2575
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates generally to the field of high content screening of particles, e.g., cells in a flow cytometric system. In particular, the present invention relates to devices, methods and systems to obtain line-scan images of particles, e.g., cells, of a plurality of different samples simultaneously, where the line-scan images can be used to identify cells based on at least one of a variety of phenotypic characteristics such as shape, asymmetry, and intracellular information for cell sorting and selection. In some embodiments, the line-scan images are obtained as the particles, e.g., cells, in a plurality of different samples flow through a plurality of microchannels, reducing the need and time for focusing of the image detection system. In some embodiments, the laser spot size has a small spatial resolution for rapid capturing of images of cells. In some embodiments, the laser spot size has a larger spatial resolution for imaging of larger particles or cells, e.g., rare cells in a sample. In some embodiments, the devices, methods and systems are automated for a high-throughput, high content screening of particles such as cells in a plurality of samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.