Methods of isothermal amplification using blocked primers
US8936921B2 · kind B2 · utility
1Cited by
6References
22Claims
0Family size
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Key dates
| Filing date | Jun 3, 2013 |
| Grant date | Jan 20, 2015 |
| Priority date | — |
| Expiry date | Jun 3, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the rpoB gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.