Reagent for blood analysis and method of use thereof
US8940499B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 16, 2009 |
| Grant date | Jan 27, 2015 |
| Priority date | — |
| Expiry date | Oct 11, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1402
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure provides a reagent for blood analysis which may include: (1) a compound having the general formula I as a fluorescent dye, wherein n, X, R1, R2, R3, R4, R5 and Y− are as defined in the specification; (2) a surfactant selected from cationic surfactants, zwitterionic surfactants and anionic surfactants. The present disclosure also provides a method to perform blood analysis including the following steps of: (a) mixing the blood sample with the reagent for blood analysis disclosed to form a cell suspension; (b) detecting the scattered light signals and fluorescence signals from the cells; and (c) differentiating and counting the cells in the blood in terms of the scattered light signals and fluorescence signals.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.