High-sensitive fluorescent energy transfer assay using fluoresent amino acids and fluorescent proteins

US8940506B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jiayu Liao · Carlsbad, US
• Yang Song · Kunming, CN
• Yongfeng Zhao · 安丰镇, CN
• Yan Liu · Nashville, US

Key dates

• Filing date: Mar 21, 2009
• Grant date: Jan 27, 2015
• Expiry date: Aug 31, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/001
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The disclosure provides method and composition utilizing fluorescent amino acids and endogenous fluorescent proteins comprising a moiety capable of undergoing FRET. The methods and compositions of the disclosure are useful in analyzing protein structure and function, and screening molecular inhibitors.