Production of hetero-oligomeric proteins in plants

US8940963B2 · kind B2 · utility

Assignee

• Icon Genetics GmbH · DE

Inventors

• Anatoly Giritch · Halle (Saale), DE
• Sylvestre Marillonnet · Bodenwerder, DE
• Victor Klimyuk · Leipzig, DE
• Yuri Gleba · Berlin, DE

Key dates

• Filing date: Jan 27, 2006
• Grant date: Jan 27, 2015
• Expiry date: Jan 11, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8258
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus-sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non-competing viral vectors.