Aptamer-based lateral flow assay and associated methods
US8945838B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 14, 2013 |
| Grant date | Feb 3, 2015 |
| Priority date | — |
| Expiry date | May 14, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/946
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods, assays, and products for the detection of analytes in a sample are provided. In one aspect, for example, a device for detecting an analyte in a sample can include a fluid transfer membrane further including a sample input region operable to receive a liquid sample, a reagent region including a first split aptamer segment, a second split aptamer segment, and a detection marker, where the first and second split aptamers are operable to ligate in the presence of the analyte. The detection marker is operable to bind to the second split aptamer. The device can further include a test region having an immobilized binding reagent operable to bind to the first split aptamer segment such that the detection marker is held in the test region when the first split aptamer segment is ligated to the second split aptamer segment due to the analyte being present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.