Functional imaging of cells with optical projection tomography

US8947510B2 · kind B2 · utility

Assignee

• VisionGate, Inc. · US

Inventors

• Michael Meyer · Aachen, DE
• J. Richard Rahn · Sammamish, US
• Anna V. Tourovskaia · Mountlake Terrace, US
• Julia Yu · Kirkland, US
• Christy A. Lancaster · Seattle, US
• Thomas Neumann · Munich, DE
• Mark E. Fauver · Seattle, US

Key dates

• Filing date: Jun 8, 2009
• Grant date: Feb 3, 2015
• Expiry date: Feb 24, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1445
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).