Detection method for sensor membrane of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes
US8951403B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 23, 2012 |
| Grant date | Feb 10, 2015 |
| Priority date | — |
| Expiry date | Dec 27, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/227
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60° C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 μl of 98.7 wt % of APS and 50 μl of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 μl of 1×PBS buffer solution, uniformly mixing same, adding 100 μl of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37° C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTixOy; and taking a measurement.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.