Multimer glycosylated nucleic acid binding protein conjugates and uses thereof
US8951736B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 25, 2012 |
| Grant date | Feb 10, 2015 |
| Priority date | — |
| Expiry date | Apr 25, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/44
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The technology relates in part to multimer conjugates comprising a scaffold linked to two or more polypeptides that specifically interact with a nucleic acid containing beta-D-glucosyl-hydroxymethylcytosine or beta-D-glucosyl-hydroxymethyluracil. The scaffold can be chosen from an antibody, an antibody fragment, a multimerized binding partner that interacts with a binding partner counterpart in each of the polypeptides, a polymer, and a polyfunctional molecule. The polypeptides can be from a kinetoplastid flagellate organism and may comprise a full-length native or modified protein or a fragment thereof that specifically interacts with the beta-D-glucosyl-hydroxymethylcytosine and/or the beta-D-glucosyl-hydroxymethyluracil in the nucleic acid. The conjugates provided herein can be used to detect the presence, absence or amount of beta-D-glucosyl-hydroxymethylcytosine and/or beta-D-glucosyl-hydroxymethyluracil-containing nucleic acid in a sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.