Method for selective labeling of protein produced by in vitro translation by using a marker from tRNA prepared by using in vitro transcription
US8951757B2 · kind B2 · utility
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Key dates
| Filing date | Jan 7, 2009 |
| Grant date | Feb 10, 2015 |
| Priority date | — |
| Expiry date | Mar 20, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/532
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method for the preparation of a labeling agent derived from one type of tRNA prepared by in vitro transcription, which is usable for non-radioactive selective labeling of target proteins produced by in vitro translation with fluorophore or biotin; and a method for selective labeling of target proteins produced by in vitro translation using the labeling agent without labeling pre-existing proteins in the reaction mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.