Retinal pigment epithelial cells differentiated from embryonic stem cells with nicotinamide and activin A
US8956866B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 27, 2008 |
| Grant date | Feb 17, 2015 |
| Priority date | — |
| Expiry date | Jun 4, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/52
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGFβ superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGFβ superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.