Nanopore and carbon nanotube based DNA sequencer

US8961757B2 · kind B2 · utility

Assignees

• ARIZONA BOARD OF REGENTS, A BODY CORPORATE OF THE STATE OF ARIZONA ACTING FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY · US
• The Trustees of Columbia University in the City of New York · US

Inventors

• Colin Nuckolls · New York, US
• Jinyao Tang · Hong Kong, CN
• Stuart Lindsay · Tempe, US
• Jin He · Plano, US
• Peiming Zhang · Gilbert, US
• Kevin Reinhart · Chandler, US

Key dates

• Filing date: Mar 18, 2009
• Grant date: Feb 24, 2015
• Expiry date: Feb 11, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a device for analyzing the composition of a heteropolymer comprising a carbon nanotube through which the heteropolymer is driven by electrophoresis. The carbon nanotube also serves as one electrode in a reading circuit. One end of the carbon nanotube is held in close proximity to a second electrode, and each end of the carbon nanotube is functionalized with flexibly-tethered chemical-recognition moieties, such that one will bind one site on the emerging polymer, and the second will bind another site in close proximity, generating an electrical signal between the two electrodes when the circuit is completed by the process of chemical recognition.