Method for electrochemically identifying target nucleotide sequences
US8962240B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 4, 2009 |
| Grant date | Feb 24, 2015 |
| Priority date | — |
| Expiry date | Aug 26, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and assembly for electrochemically identifying target nucleotide sequences. The method includes supplying a biological sample that may contain a predetermined target nucleotide sequence; supplying activatable amplification materials comprising free nucleotides to form replicated target nucleotide sequences; supplying an oxido-reducible compound capable of being inserted during replication between the nucleotides forming the replicated target sequences; and activating the activatable amplification materials before applying an electric field to the sample in order to activate the oxido-reducible compound. The replicated target sequences cause inhibition of electrochemical activity of the inserted oxido-reducible compound, and the presence of the predetermined target nucleotide sequence is determined in instances where the electric current decreases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.