Method for providing DNA fragments derived from an archived sample

US8962246B2 · kind B2 · utility

Assignee

• EPIGENOMICS AG · DE

Inventors

• Matthias Ballhause · Berlin, DE
• Kurt Berlin · Stahnsdorf, DE
• Dimo Dietrich · Berlin, DE
• Antje Kluth Lukas · Bargteheide, DE
• Matthias Schuster · Weinheim, DE
• Ute Wagner · Berlin, DE
• Reinhold Wasserkort · Berlin, DE
• Heike Ziebarth · Berlin, DE

Key dates

• Filing date: Dec 2, 2013
• Grant date: Feb 24, 2015
• Expiry date: Dec 2, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc.). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, and are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.