Patent · US Active

Production host cell lines

US8962312B2 · kind B2 · utility

0Cited by

3References

13Claims

0Family size

Assignee

BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG · DE

Inventors

Hitto Kaufmann · Ulm, DE
Lore Florin · Biberach, DE
Eric Becker · Biberach an der Riß, DE
Joey M. Studts · Biberach, DE

Key dates

Filing date	Jul 22, 2009
Grant date	Feb 24, 2015
Priority date	—
Expiry date	Apr 8, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N9/003
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a DHFR expression cassette. Those cell lines have improved growth characteristics in comparison to DHFR-deficient or DHFR-reduced cell lines such as CHO DG44 and CHO DUKX-B11. The invention especially concerns two cell lines, a representative of each cell line is deposited with the DSMZ under the number DSM ACC2909 (CHOpper® Discovery) and DSM ACC2910 (CHOpper® Standard). The invention further concerns a method of producing proteins using the cells generated by the described method.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.