Method and computer program product for distinguishing and sorting seeds containing a genetic element of interest

US8965564B2 · kind B2 · utility

Assignee

• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Steven M. Becker · Johnston, US
• Steven J. Corak · Johnston, US
• James L. Hunter · Ankeny, US
• Gary L. Jaehnel · Des Moines, US
• Hugh Lu · Johnston, US
• James W. Stevenson · Des Moines, US

Key dates

• Filing date: Apr 25, 2013
• Grant date: Feb 24, 2015
• Expiry date: Apr 25, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/85
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker.