Patent · US Active

Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use

US8969003B2 · kind B2 · utility

0Cited by

23References

15Claims

0Family size

Assignee

Elitech Holding B.V. · NL

Inventors

Alexei Vorobiev · Redmond, US
Eugeny A. Lukhtanov · Bothell, US
Noah Scarr · Seattle, US

Key dates

Filing date	Mar 14, 2013
Grant date	Mar 3, 2015
Priority date	—
Expiry date	Mar 14, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6846
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogs can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogs can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.