Method for recovering sperm nucleic acid from a forensic sample
US8969044B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jul 22, 2008 |
| Grant date | Mar 3, 2015 |
| Priority date | — |
| Expiry date | Oct 4, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.