Patent · US Active

Method for detecting pyrophosphate by means of bioluminescence detection

US8969045B2 · kind B2 · utility

8Cited by

3References

11Claims

0Family size

Assignee

Bayer Intellectual Property GmbH · DE

Inventors

Nils Burkhardt · Velbert, DE
Stefan Heitmeier · Wülfrath, DE

Key dates

Filing date	Nov 7, 2007
Grant date	Mar 3, 2015
Priority date	—
Expiry date	Aug 15, 2031

Classification

Technology area (CPC G)Physics
CPC primaryG01N2333/988
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to methods for detecting pyrophosphate by means of bioluminescence detection. In addition, methods for measuring chemical, especially enzyme-catalyzed, reactions in which pyrophosphate is formed or consumed are described. Such reactions are catalyzed for example by guanylyl cyclases, adenylyl cyclases, DNA polymerases or RNA polymerases.The novel methods are distinguished by high sensitivity and low susceptibility to interference, can easily be automated and miniaturized and are additionally suitable for carrying out continuous measurements. The methods can be employed particularly advantageously in the area of medical diagnosis and biomedical research, including pharmaceutical active ingredient research.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.