Patent · US Active

Solid support assay systems and methods utilizing non-standard bases

US8969047B2 · kind B2 · utility

0Cited by
47References
9Claims
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Key dates

Filing dateJul 24, 2013
Grant dateMar 3, 2015
Priority date
Expiry dateJul 24, 2033

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/156
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay.

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