Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography
US8969532B2 · kind B2 · utility
23Cited by
192References
65Claims
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Key dates
| Filing date | Oct 3, 2007 |
| Grant date | Mar 3, 2015 |
| Priority date | — |
| Expiry date | Sep 1, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/09
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides processes for the manufacturing of polypeptide conjugates. In particular, the invention provides methods for the purification of polypeptide conjugates, which include at least one polymeric modifying groups, such as a poly(alkylene oxide) moiety. Exemplary poly(alkylene oxide) moieties include poly(ethylene glycol) (PEG) and poly(propylene glycol). In an exemplary process, hydrophobic interaction chromatography (HIC) is used to resolve different glycoforms of glycoPEGylated polypeptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.