Patent · US Active

Deducing exon connectivity by RNA-templated DNA ligation/sequencing

US8975019B2 · kind B2 · utility

0Cited by

16References

10Claims

0Family size

Assignee

University of Massachusetts · US

Inventors

Phillip D. Zamore · Marlborough, US
Melissa Moore · Chicago, US
Christian Roy · Quebec City, CA

Key dates

Filing date	Oct 18, 2010
Grant date	Mar 10, 2015
Priority date	—
Expiry date	Oct 6, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6869
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides an HTS-compatible read length sequence that comprises information that corresponds to the consecutive order of the exons in the original template oligonucleotide.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.