Methods for improved diagnosis of dysplasias

US8975036B2 · kind B2 · utility

Assignee

• Ventana Medical Systems, Inc. · US

Inventors

• Ruediger Ridder · Schriesheim, DE
• Anja Reichert · Schallstadt, DE
• Marcus Trunk-Gehmacher · Heidelberg, DE
• Richard Batrla · Heidelberg, DE

Key dates

• Filing date: Oct 29, 2008
• Grant date: Mar 10, 2015
• Expiry date: Jan 9, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/57484
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a method for improved diagnosis of dysplasias based on simultaneous detection of INK4a gene products and at least one marker for cell proliferation. Particularly the present invention provides a method for discriminating dysplastic cells over-expressing INK4a gene products from cells over-expressing INK4a gene products without being dysplastic by detection of a marker suitable for characterizing the proliferation properties of the respective cell. The characterization of the proliferation properties may comprise the detection of a marker or a set of markers characteristic for active cell proliferation and/or a marker or a set of markers characteristic for retarded or ceased cell proliferation. The method presented herein thus enables for a specific diagnosis of dysplasias in histological and cytological specimens.