Method for generating aptamers with improved off-rates

US8975388B2 · kind B2 · utility

Assignee

• SomaLogic, Inc. · US

Inventors

• Dominic Zichi · Boulder, US
• Sheri K. Wilcox · Longmont, US
• Chris Bock · Durham, US
• Daniel J. Schneider · Arvada, US
• Bruce Eaton · Boulder, US
• Larry Gold · Boulder, US

Key dates

• Filing date: May 23, 2011
• Grant date: Mar 10, 2015
• Expiry date: Sep 26, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2330/31
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.