Method of identifying a candidate compound which may inhibit α9-nAchR overexpression or estrogen receptor-dependent transcription in nicotine-derived-compound-induced breast cancer cells
US8980571B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 2011 |
| Grant date | Mar 17, 2015 |
| Priority date | — |
| Expiry date | Oct 25, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/944
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to methods of identifying a candidate compound which may inhibit estrogen receptor-dependent transcription or α9-nAChR overexpression and proliferation of nicotine-derived-compound-induced breast cancer cells by using an activating protein 1 (AP1) polypeptide. The invention found that α9-nAChR has an activating protein 1 (AP1)-binding site, that the α9-nAChR promoter is located at the AP1-binding site, and that ERs specifically bind to the α9-nAChR promoter at the AP1-binding site, indicating that ER-induced α9-nAChR up-regulation plays a central role in the response to endogenous (E2) or exogenous (nicotine) stimulation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.