Patent · US Active

Mutant glycerol dehydrogenase (GlyDH) for the production of a biochemical by fermentation

US8980604B2 · kind B2 · utility

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11Claims
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Assignee

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Key dates

Filing dateJul 30, 2010
Grant dateMar 17, 2015
Priority date
Expiry dateMay 27, 2031

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N9/0006
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced.The present invention also relates to a mutant glycerol dehydrogenase (GlyDH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein

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