Composition, method and kit for obtaining purified recombinant proteins
US8981067B2 · kind B2 · utility
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3References
12Claims
0Family size
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Key dates
| Filing date | Aug 26, 2011 |
| Grant date | Mar 17, 2015 |
| Priority date | — |
| Expiry date | Mar 21, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y601/01007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions relating to a combination of two types of separation matrix; and to variant host cells which contain at least one essential host protein that is fused to an affinity binding tag or has been mutated to replace at least two of a plurality of histidines or basic amino acids are provided. Methods are also provided that relate to isolating a recombinant protein from a lysate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.