Method for evaluating specific incorporation of D-glucose into cells
US8986656B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 7, 2009 |
| Grant date | Mar 24, 2015 |
| Priority date | — |
| Expiry date | Aug 10, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/533
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.