Sequencing reactions with alkali metal cations for pulse width control

US8986930B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Andrei G. Fedorov · Atlanta, US
• John Lyle · Fremont, US
• Keith Bjornson · Fremont, US
• Jeremiah Hanes · Woodside, US

Key dates

• Filing date: Jul 7, 2011
• Grant date: Mar 24, 2015
• Expiry date: Oct 4, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.