Microorganisms with extended substrate utilization range

US8986960B2 · kind B2 · utility

Assignee

• NESTE OIL OYJ · FI

Inventors

• Shanna Sichwart · Pforzheim, DE
• Birgit Haschenhermes · Meppen, DE
• Daniel Broker · Münster, DE
• Alexander Steinbuchel · Havixbeck, DE
• Stephan Hetzler · Munster, DE
• Perttu Koskinen · Helsinki, FI

Key dates

• Filing date: Jul 15, 2011
• Grant date: Mar 24, 2015
• Expiry date: Nov 2, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P7/625
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are microorganisms of the genus Cupriavidus or Ralstonia, which are genetically modified to express phosphomannose isomerase (EC5.3.1.8) and facilitated diffusion protein (EC1.3.1.74) for mannose uptake, and optionally mannofructokinase (EC2.7.1.4). The microorganisms also may be genetically modified to express xylose isomerase (EC 5.3.1.5), xylulokinase (E 2.7.1.17) and xylose proton symporter E or a high affinity ABC-transporter. The genetically modified microorganisms are capable of growing on mannose, xylose, arabinose, glucose, or galactose, or a combination thereof as the carbon source.