Compositions and methods for labeling and imaging phospholipids
US8987514B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Feb 4, 2010 |
| Grant date | Mar 24, 2015 |
| Priority date | — |
| Expiry date | Jan 26, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a method to label phospholipids in vivo based on the metabolic incorporation of an alkynyl- or azido-labeled metabolic precursor into phospholipids. The resulting phospholipids have alkynyl or azido moieties, which, upon reaction with a labeled azide or alkyne, respectively, form labeled compounds that can be visualized using optical or electron microscopy with high sensitivity and spatial resolution in cells or tissue. The present method provides a valuable tool for imaging phospholipid synthesis, turnover and subcellular localization in cultured cells as well as in animals.
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