Unshrunken tissue equivalent and methods for producing such an unshrunken tissue equivalent
US8992613B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 1, 2010 |
| Grant date | Mar 31, 2015 |
| Priority date | — |
| Expiry date | Aug 24, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2535/10
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
The invention relates to a method for producing an unshrunken tissue equivalent. Said method is comprising in that: a mixture is produced that contains at least one element of an extracellular matrix; at least one pluridimensional medium is soaked with said mixture; from the components of the mixture, a lattice comprising at least one element of an extracellular matrix is produced, at least at the medium; at least part of the components of said mixture is attached to the structure of the medium; the shrinking of at least the lattice is prevented and at least said lattice is tensioned on said medium; fibroblasts are integrated into the lattice and at least one cell culture of said fibroblasts is carried out, at least in the lattice. The invention also relates to a method for producing a skin equivalent that comprises at least one dermal equivalent formed by an unshrunken tissue equivalent and at least one epidermal equivalent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.