Preparation and uses of gene sequences encoding chimerical glycosyltransferases with optimized glycosylation activity
US8993297B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 24, 2007 |
| Grant date | Mar 31, 2015 |
| Priority date | — |
| Expiry date | Jan 17, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/05
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the production of gene sequences encoding chimerical membrane glycosyltransferases presenting an optimized glycosylation activity in cells transformed with the sequences, the gene sequences corresponding to the fusion: of a first nucleic acid coding for a C-terminal minimal fragment of the catalytic domain (CD) of the native full length glycosyltransferase, to a second nucleic acid coding for a transmembrane peptide comprising in its N-terminal region a cytoplasmic tail (CT) region located upstream from a transmembrane domain (TMD), itself located upstream of a stem region (SR), provided that at least one of these CT, TMD, SR peptides being different from the primary structure of the naturally occurring peptide counterparts present in the native glycosyltransferase from which is derived the CD fragment with optimal glycosyltransferase activity as defined above.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.